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Veterinary Immunohistochemistry (IHC) Assay Development
Immunohistochemistry (IHC) holds a significant position within veterinary medicine, serving as a potent technique to analyze protein expression and biomolecule localization in animal tissues. The upsurge in the requirement for veterinary IHC services underscores its importance. Acknowledging growing need of IHC assay development to explore diverse animal diseases and health conditions, BioVenic has taken a stride by introducing an all-encompassing service dedicated to the development of veterinary IHC methodologies.
What Is IHC?
The technique of IHC utilizes antibodies to meticulously label specific proteins and other biomolecules within tissue samples. This versatility in IHC's application extends to its capability to discern a wide array of biomolecules, including antigens, enzymes, hormones, and receptors. Far beyond its diagnostic efficacy, IHC emerges as a precious instrument for delving deep into the complexities of regular physiological processes, spanning from tissue maturation to immune responses.
Fig. 1 Immunohistochemistry detection steps and schematic diagram.1
Veterinary IHC Assay Development Services
BioVenic offers comprehensive veterinary IHC development services for various species, including but not limited to, dogs, cats, horses, and cattle. Our services include antibody preparation, antigen retrieval, blocking, primary and secondary antibody incubation, detection, and visualization of specific targets. We use a range of detection systems, including chromogenic, fluorescent, and enzyme-based detection systems, to produce high-quality images and reliable results. Our customized services can fully meet the needs of customers in terms of targets, substrates, fluorescent dyes, counterstains, etc. Some of the targets for IHC assay development are shown below. ▼
We provide a variety of substrates, counterstains, and antigen retrieval buffers, and will determine the most suitable counterstain/substrate combination according to your research purpose. The table below shows some of the options. ▼
Veterinary Immunohistochemistry (IHC) Development Process
We provide consulting services to discuss target molecules, animal species, and tissue types of interest, and the level of sensitivity and specificity required for detection.
We select and validate the most appropriate primary and secondary antibodies for the IHC assay. We use a range of techniques to validate the antibodies.
We optimize the staining conditions for the IHC assay, including the antibody dilution, the incubation time, and the chromogen or fluorophore used for detection. We use a range of techniques to optimize the staining conditions, including titration experiments and the use of positive and negative control samples.
We help customers validate the IHC assay by testing it on a range of animal tissue samples, including both normal and diseased tissues. We use a range of techniques to validate the assay, including visual inspection, image analysis, and statistical analysis.
Applications
- IHC is used to diagnose various cancer markers, and prognostic markers, and to detect the origin, staging, grading, and prognosis of cancer in research animals.
- IHC studies animal infectious diseases and monitors animal health by diagnosing animal bacteria, viruses, and other pathogens.
Why Choose Us?
We help choose the optimal sample handling method to preserve antigenicity based on the tissue type being studied, the antigen of interest, etc.
We assist to select and prepare antibodies considering multiple factors, including target antigens, animal species, and downstream applications.
We optimize the staining protocol based on the properties of the target and image the stained tissue using immunofluorescence or confocal microscopy for clear results.
BioVenic has comprehensive proficiency in all aspects of the IHC development process, including antibody selection and optimization. Our veterinary IHC assay development services satisfy customers' diagnostic research on animal infectious diseases, tumors, and other diseases. If any questions or concerns arise, we encourage you to contact us for assistance.
References
- Lovchik, Robert D., et al. "Rapid micro-immunohistochemistry." Microsystems & nanoengineering 6.1 (2020): 94.