Animal Embryonic Stem Cell Culture Development

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Animal Embryonic Stem Cell Culture Development

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Background Comparison of Commonly Used Animal ESC Culture Systems Media Formulation Development Culture Condition Optimization Cell Contamination Control Why Choose Us?

BioVenic conducts comprehensive and in-depth research and customized service development on advanced culture systems for in vitro research and induced differentiation of animal stem cells. Our research team supports the development of various standardized culture systems, such as feeder-layer culture systems, feeder-free systems, and serum-free systems. These systems are designed to support the continuous division and proliferation of ESCs while maintaining their undifferentiated state. We assist researchers in overcoming the challenges of ESC culture by helping them find a culture method that better suits their experiments and by selecting the most optimized embryonic stem cell culture medium to support their applications of ESCs.

Background

Animal embryonic stem cells are highly undifferentiated pluripotent cells that can be isolated from the inner cell mass of early embryos and cultured in vitro using an appropriate culture system. ESCs are naturally immortalized cells with a permanent self-renewal capacity. They maintain an undifferentiated state, exhibit high telomerase activity, have a normal karyotype, express embryonic surface markers, and express pluripotent transcription factors under appropriate culture conditions. Animal ESCs provide researchers with an ideal biological platform. This platform allows scientists to conduct a wide range of scientific research. These include establishing veterinary drug screening platforms, studying molecular regulatory mechanisms of animal cell development and differentiation, constructing gene-targeted knockout animal models, and developing gene therapy vectors for cell therapy and regenerative medicine.

Fig.1 Animal Embryonic Stem Cell Isolation and Culture. (Hou, et al., 2018) Fig.1 Flow Chart of Animal Embryonic Stem Cell Isolation and Culture.^1,2

Comparison of Commonly Used Animal ESC Culture Systems

Systems	Feeder Layer Culture Method	Feeder-free Culture Method	Complete Medium Culture Method
Advantages	It provides ESC with complex protein molecules necessary for stem cell contact.	Its preparation is simple, operation is easier, and culture time is saved.	It promotes stem cell proliferation, inhibits stem cell differentiation, maintains stem cell phenotype, and increases the number of stem cell passages.

Media Formulation Development

BioVenic offers customized media formulation design and development services to scientists engaged in animal stem cell research projects involving embryonic stem cells from various species. We utilize our extensive experience and professional background in animal cell biology and molecular biology, along with our equipment resources, to ensure the successful completion of precise in vitro culture and induced differentiation of animal embryonic stem cells using culture media. We create reliable formulas for our customers that precisely meet the requirements of embryonic stem cells and ensure that their pluripotency and cell viability are maintained.

Culture Condition Optimization

BioVenic offers customized services to optimize culture programs for in vitro proliferation and directed differentiation, tailored to the client's animal embryonic stem cell culture programs. The feeder layer culture system is the most widely used technical method for establishing ESC in vitro culture systems and plays a crucial role in maintaining ESC self-renewal and pluripotency. Our dedicated team has extensively researched the mechanism of the feeder layer culture system, thoroughly understood the influencing factors, and subsequently provided customized optimization strategies. By adjusting key parameters such as the ratio of growth factors and culture time, we can develop conditioned media that are more complex than traditional culture media. This customization can be tailored to the customer's individual project goals, ensuring efficient proliferation or directed differentiation of ESCs in vitro and facilitating the smooth running of the experiment.

Cell Contamination Control

BioVenic effectively addresses common contamination issues in the in vitro cell culture process of biological laboratories. The maintenance of ESC pluripotency largely depends on the extracellular microenvironment to provide appropriate signals, and maintaining strict quality control of the microenvironment is critical. For all cell culture-related services, we help customers overcome and prevent the negative consequences associated with contamination, such as changes in cell state, reduced reproducibility of experimental results, and potential cell death. We closely monitor contamination to ensure strict quality control of animal ESC and culture-related products, as well as customized solutions. At the same time, we use contamination control platforms to detect and optimize bacteria, fungi, and mycoplasma in culture systems. This ensures the correct pH, osmotic pressure and endotoxin-free environment.

Cell Contamination	Mycoplasma Contamination	Slow growth rate, decreased vitality, and easy detachment. Cell morphology changes. During the cultivation process, the culture medium quickly turns yellow, indicating a high concentration of invisible microorganisms multiplying in the culture liquid and consuming nutrients. Under the microscope, a large number of debris is visible in the culture medium that is not turbid. Cells tend to aggregate.
	Bacterial Contamination	Under the microscope, a large number of spherical particles can be seen floating in the culture medium. The cell culture medium quickly becomes cloudy in a short period of time. The culture medium does not appear turbid when left standing, but a significant amount of turbid matter floats up when slightly shaken. Cells stop growing and die in large numbers.
	Fungal Contamination	Under a microscope, you can observe crisscrossing filamentous, dendritic, or tubular hyphae between cells or in the culture medium. Light yellow or white floating objects will form in the cell culture medium, which are visible to the naked eye, but the culture medium will not become cloudy in the short term. Most hyphae can be observed in chain-like arrangements under high magnification, while Candida and yeast strains appear oval and scattered on and around the cells.

Why Choose Us?

The comprehensive development of diversified culture solutions ensures that customers' individual needs for various applications of animal embryonic stem cells are met.

The stringent cell culture media quality control system accurately manages the quality of each culture system development solution.

From the development of culture systems to the design and optimization of media solutions, we offer a comprehensive service tailored to the specific experimental requirements of our customers.

BioVenic offers a comprehensive range of services for the development and customization of animal embryonic stem cell culture systems. This includes a selection of high-quality complete culture media for in vitro expansion and differentiation. Our research and development team specializes in tailoring solutions to the scientific challenges faced by researchers working with different animal ESCs. We provide customized growth and differentiation culture protocols for embryonic stem cells. For more information about our animal ESC culture development solution, please contact us today!

References

Image retrieved from Figure 3 "A schematic of ESC derivation and in vitro induced gametogenesis." Hou et al., 2018, used under [CC BY 4.0] (https://creativecommons.org/licenses/by/4.0/). The original image was modified by extracting and using only part b, and the title was changed to "Flow Chart of Animal Embryonic Stem Cell Isolation and Culture."
Hou, Zhuocheng, et al. "Revolutionize livestock breeding in the future: an animal embryo-stem cell breeding system in a dish." Journal of animal science and biotechnology 9 (2018): 1-11.