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Feed Postbiotics-Cell Lysates
BioVenic provides feed postbiotics (microbial cell lysate) development services to create safe and effective cell-lysate supplements that enhance animal growth, immunity, and product quality, supporting feed postbiotic development and animal nutrition research. Our platform covers microbial cell lysate production via mechanical, chemical, and enzymatic methods, with analysis of bioactive components (polysaccharides, peptides, nucleotides, vitamins, SCFAs) via GC-MS, HPLC, and NMR, alongside comprehensive safety and efficacy evaluation. We support cell-lysate validation with in vitro and in vivo models across livestock, poultry, aquaculture, and companion animals, and multi-omics (microbiome, metabolome, proteome) analyses to elucidate mechanisms and application effects.
Feed Postbiotics Cell Lysates Analysis
Microbial cell lysates encompass a mix of biologically active compounds with diverse roles. Our feed analysis tools aid in assessing common dietary compositions like proteins, fats, and carbohydrates within these microbial cell lysates. Leveraging advanced techniques such as GC-MS, HPLC, and NMR, we pinpoint and evaluate the functional constituents. High-throughput metabolite analysis technologies assist in identifying specific metabolites and uncovering new ones. Moreover, our platform enables the customization of targeted assays for specific components within the microbial cell lysates.
In Vivo and In Vitro Evaluation of Cell Lysates
- Feed Postbiotics Cell Lysates Safety Evaluation
Given their microbial origins, it's crucial to verify the safety of cell lysates both in vivo and in vitro. We can assess the genetic safety of microorganisms and detect potential contaminants like endotoxins, mycotoxins, and heavy metals. Our animal models are capable of evaluating the safety of these microbial cell lysates by observing changes in appearance and biochemical indicators.
Table. 1 Safety concerns about microbial cell lysates
| Safety concerns | Description |
|---|---|
| Pathogenic Contamination | Verifying absence of disease-causing organisms in microbial cell lysates. |
| Endotoxins | Monitoring and controlling levels of Lipopolysaccharides (LPS) from gram-negative bacteria to prevent septic shock risks. |
| Allergenicity | Identifying allergenic factors in microbial lysates to avert allergic reactions in animals. |
| Toxins | Assessing for presence of harmful toxins or mycotoxins in microbial cell lysates to safeguard animal health. |
| Antibiotic Resistance | Mitigating risks of transmitting antibiotic resistance genes to animal gut microbiomes or the environment. |
| Heavy Metal Contamination | Evaluating levels of heavy metals in microbial cell lysates during cultivation and processing to ensure safety. |
| Dosage | Establishing optimal dosages for maximizing benefits while minimizing adverse effects. Long-term effects on animal health and product quality are also a focus of study. |
- Feed Postbiotics Cell Lysates Efficacy Evaluation
Microbial cell lysates can exhibit biological activities such as immunomodulation, antioxidant, and antibacterial effects. Using various in vitro and in vivo models, combined with technologies like metabolomics, microbiome analysis, and proteomics, we offer tailored solutions for evaluating these effects in contexts critical to animal nutrition research.
Mechanism Study of Feed Postbiotics Cell Lysates
Our mechanism study services integrate animal model research with microbial lysate analysis to provide comprehensive insights into single metabolites, genomes, microbiomes, proteomes, and metabolomes. This approach helps researchers understand how different cell lysates impact animal nutrition and health by correlating various indicators with genomic data.
Table. 2 Bioactive components in microbial cell lysates
| Bioactive Components | Examples |
|---|---|
| Polysaccharides | β-Glucan, mannan, trehalose |
| Bioactive Peptides | Antioxidant peptides, antimicrobial peptides |
| Nucleotides | Yeast nucleotides |
| Vitamins | Vitamin B12, vitamin B6 |
| Short Chain Fatty Acids | Acetate, propionate, butyrate, pentanoate, malonate |
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Feed Cell Lysate Postbiotics Development
Microbial cell lysates are generated through mechanical force, chemical treatments, or enzymatic digestion. Our innovations optimize the enzymatic production method, increasing its effectiveness for creating animal postbiotics. Each microbial strain undergoes individual production, harvesting, specific inactivation treatment, and optional lyophilization. The synthesis of a polyvalent microbial cell lysate involves blending distinct lysates in preset proportions.
Effects of Feed Postbiotics Cell Lysates on Animals
Microbial cell lysates contain various beneficial compounds like peptides, enzymes, vitamins, and other metabolites. These have the following potential effects on animals:
| Effects | Description |
|---|---|
| Gut Health Improvement | Enhances gut microbiome diversity, strengthens intestinal barriers, reduces inflammation, and promotes beneficial gut microbes. |
| Immune System Modulation | Stimulates immune responses, increases antimicrobial peptides and cytokines, improves pathogen resistance. |
| Growth Performance Enhancement | Boosts growth rates, improves feed conversion efficiency, enhances overall productivity, and positively influences nutrient utilization. |
| Pathogen Inhibition | Exhibits antimicrobial properties, reduces infection risks and antibiotic needs. |
| Stress Reduction | Lowers stress levels and enhances animal resilience, improving welfare and productivity. |
| Egg and Milk Quality Improvement | Enhances quality and nutritional profile of eggs and milk in laying hens and dairy animals. |
Related Research
In a study assessing the effects of postbiotics and paraprobiotics (heat-inactivated Lactiplantibacillus plantarum cells) as antibiotic replacements on broiler chicken performance, 360 one-day-old chicks were assigned to six treatment groups: negative control, positive control (antibiotic), postbiotic cell-free supernatant, paraprobiotic whole-cell lysate, combined postbiotic + paraprobiotic, and commercial probiotic, over a 42-day trial. Results showed that the paraprobiotic cell lysate group achieved comparable growth performance to the antibiotic-positive control, with body weight gain of 2,285 g vs. 2,310 g and feed conversion ratios of 1.52 vs. 1.50, respectively (p > 0.05), while both significantly outperformed the negative control (p < 0.05). Small intestine histomorphology revealed that paraprobiotic supplementation significantly increased villus height and villus-to-crypt ratio in the jejunum and ileum compared to the negative control. Immune status analysis demonstrated that the paraprobiotic group had significantly higher serum IgG and IgA titers, along with increased bursal and thymus weights indicating enhanced lymphoid organ development. Hepatic growth gene expression analysis showed that paraprobiotic treatment upregulated IGF-1 and GHR mRNA levels in liver tissue, providing a molecular basis for the observed growth promotion. This study establishes that heat-inactivated microbial cell lysates (paraprobiotics) serve as effective antibiotic alternatives in broiler production by simultaneously improving growth performance, intestinal morphology, immune competence, and growth-related gene expression.
Fig. 1 Villi heights and crypt depth of small intestines.1
Why Choose Us?
We provide advanced omics technologies and data analysis services to study microbiota, metabolites, and proteins for applying microbial cell lysates across various animals.
Our in vivo and in vitro models cater to a broad range of animals, essential for thorough safety and efficacy evaluations of microbial cell lysate feed postbiotics.
Our assay platforms are adept at detecting numerous bioactive components in cell lysates, and we can develop specific assays to meet research needs.
Case Study
Researchers developed and characterized a polyvalent microbial cell lysate postbiotic through optimized enzymatic digestion of selected probiotic strains, followed by comprehensive compositional profiling and functional activity assessment. The lysate production process employed strain-specific enzymatic inactivation with controlled lyophilization to preserve bioactive integrity, and individual lysates were blended at optimized ratios to create a multi-functional postbiotic formulation. The sunburst composition analysis revealed that polysaccharides—predominantly β-glucan (22%) and mannan (15%)—constituted the largest component class (45% total), followed by bioactive peptides including antioxidant and antimicrobial species (28%). Nucleotides, B-vitamins, and SCFAs contributed the remaining bioactive fraction. The functional activity profile demonstrated strong gut health support (92) and immunomodulatory capacity (88), with substantial antioxidant (82) and antimicrobial (75) activities—directly correlating to the identified β-glucan, bioactive peptide, and SCFA constituents. This polyvalent cell lysate postbiotic subsequently confirmed efficacy in vivo across multiple target animal species, showing enhanced growth performance, improved intestinal barrier function, reduced pathogen colonization, and elevated immune markers without the safety concerns associated with live probiotic administration.
Fig. 2 Case Study
Frequently Asked Questions
Q1. What types of cell lysate postbiotics can be developed through your service platform?
A: Our service supports the development and processing of various cell lysate postbiotics derived from beneficial microorganisms such as lactic acid bacteria and yeasts. We utilize advanced disruption techniques to release intracellular bioactive components, including peptides, peptidoglycans, and organic metabolites, tailored for livestock and poultry feed applications. If you have specific microbial strains or unique formulation targets in mind, please consult our technical support team to confirm feasibility.
Q2. What are the sample requirements for initiating a feed cell lysate project with your team?
A: Typically, we require sufficient quantities of your target microbial biomass, viable cultures, or preliminary raw fermentation broths, along with detailed documentation regarding strain safety and handling guidelines. The exact physical state and concentration depend on whether you are scaling up an existing process or starting from raw strain characterization. For detailed shipping conditions, quantity minimums, and specific preservation protocols, we strongly recommend reaching out directly to our technical support staff before sending samples.
Q3. How do feed-grade cell lysates differ from live probiotics in terms of stability and application?
A: Cell lysates represent non-viable microbial cell preparations containing inactivated cellular fractions and released metabolites. Unlike live probiotics, they exhibit superior heat and pressure tolerance, making them exceptionally stable during high-temperature feed pelleting, extrusion, and long-term storage processes. This robust stability ensures consistent biological efficacy within complex feed matrices. If you need assistance selecting the ideal processing parameters for your specific feed manufacturing line, feel free to contact our technical support.
With our comprehensive range of services, including feed analysis, safety and efficacy evaluations, mechanistic research, and product development solutions, BioVenic offers a one-stop solution for developing microbial cell lysates as feed postbiotics for animals. If you're interested in our cell lysate feed postbiotics solutions, please contact us to discuss your research program further.
Reference
- Danladi, Yohanna, et al. "Effects of postbiotics and paraprobiotics as replacements for antibiotics on growth performance, carcass characteristics, small intestine histomorphology, immune status and hepatic growth gene expression in broiler chickens." Animal12.7 (2022): 917. https://doi.org/10.3390/ani12070917. Distributed under Open Access license CC BY 4.0. Without modification.