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Natural Carbohydrate-based Veterinary Vaccine Development
Cell surface polysaccharides and glycoconjugates from bacteria have emerged as valuable antigenic targets for veterinary vaccine development and have been widely used to prevent several animal infectious diseases. BioVenic has extensive experience in providing natural carbohydrate-based veterinary vaccine development services, helping you protect animals against many different types of pathogens.
Fig.1 Bacterial polysaccharides can be used as vaccine antigens.1
Our Services
Bacteria Culture
BioVenic provides specialized bacteria culture services for natural carbohydrate-based veterinary vaccines to support your veterinary vaccine development research and projects. Our services cover multifaceted polysaccharide production strain culture needs to ensure high-quality polysaccharide production. Services include maintaining and amplifying your starting strains to meet your development needs. During this process, we select the appropriate pathogen media for you, providing a safe and clean culture environment. We also provide support in the development of customized media formulations and optimization of fermentation conditions to improve polysaccharide yields.
Polysaccharide Extraction
Polysaccharides need to be extracted from sufficient amounts of usable bacteria as natural carbohydrate-based veterinary vaccine components. BioVenic offers polysaccharide extraction services, covering every step of the process, to help you develop your veterinary vaccines.
Sterilization
BioVenic usually uses pasteurization for sterilization, and due to the higher temperature of this method, it also improves the solubility of polysaccharides and reduces the viscosity of the solution, which facilitates subsequent centrifugation or filtration.
Crude Extraction
Specific steps include generally raw material degreasing, decolorization, extract concentration, and precipitation with ethanol, acetone, and other precipitants. The precipitation part can be repeated several times to dissolve and re-precipitation.
Purification
BioVenic provides purification services to remove small molecule impurities and proteins in the crude polysaccharides. Methods include Protease, Sevag, TCA, and Trifluorotrichloroethane methods, etc. For the subsequent separation, we provide ion exchange chromatography and gel column chromatography.
Polysaccharide Characterization
BioVenic offers polysaccharide characterization services designed to provide insight and assessment of the nature, structure, and function of polysaccharides, facilitating the assurance of batch-to-batch consistency and stability of natural carbohydrate-based veterinary vaccines you develop.
| Polysaccharide Characterization Services | Analysis Platforms |
|---|---|
| Monosaccharide composition | GC, HPLC, HPAEC |
| Composition of the main chain and branches | HPLC, HPAEC, MALDI-TOF |
| Judgment of sugar ring and glycoside bonds | GC, GC-MS |
| Connection of glycosidic linkages | GC-MS |
| Determination of purity and molecular weight | HPGPC, HPSEC-MALLS |
| Judgment of characteristic | FT-IR |
| Anomeric configuration and the connection of sugar residue | 1D/2D NMR |
| Micromorphology | AFM |
| Zeta potential and hydrodynamic radius | Nano-Zetasizer |
Natural Carbohydrate-based Veterinary Vaccine Development Services
| BioVenic provides formulation development services tailored to your needs, offering suitable adjuvant selection and adjuvant development services. Drawing from our past project experiences, we optimize adjuvants and other components within the formulation to enhance the immunogenicity of polysaccharide vaccines. Our immunogenicity assessment ensures that you attain higher levels of immunogenicity. | During the polysaccharide extraction process, there is also a risk of bacterial endotoxin residue. BioVenic offers endotoxin residue detection services to ensure that endotoxin levels in vaccine samples comply with safety standards. This service is an integral part of our quality control within veterinary vaccine development services, which empowers you to develop high-quality and highly stable natural carbohydrate-based veterinary vaccines. | As a subtype of subunit vaccines, natural carbohydrate-based veterinary vaccines are known for their high safety profile. However, compared to traditional live attenuated bacteria veterinary vaccines, they often exhibit relatively lower immunogenicity. We provide in vitro and in vivo immunogenicity and immunotoxicity assessment for natural carbohydrate-based veterinary vaccine development. |
Why Choose Us?
BioVenic optimizes the bacterial cultivation environment and techniques to achieve high yields and purity of raw materials.
BioVenic has established multiple technological platforms for your use in polysaccharide characterization.
BioVenic enforces strict quality control in your natural carbohydrate-based veterinary vaccine development, ensuring the consistency and stability of vaccines.
Natural carbohydrate-based veterinary vaccines represent a category of vaccines with a long history of use. BioVenic possesses extensive experience in providing services for such projects, assisting numerous researchers in accomplishing development. the vaccine development for livestock, poultry, companion animals, aquaculture species and more. Contact us now to confront the challenges of natural carbohydrate-based veterinary vaccine development together, and let our services facilitate the achievement of your goals!
References
- Gómez-Redondo, Marcos, et al. "Bacterial polysaccharides: conformation, dynamics and molecular recognition by antibodies." Drug Discovery Today: Technologies 35 (2020): 1-11.
- Huang, Xiaojun, et al. "Guideline for the extraction, isolation, purification, and structural characterization of polysaccharides from natural resources." eFood 3.6 (2022): e37.