Point Mutation Introduction in Precision Animal Breeding

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Point Mutation Introduction in Precision Animal Breeding

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Point mutations occur in the genome when a single base pair is added, deleted, or changed. They can have various functional consequences, including changes in gene expression or alterations in encoded proteins. Introducing point mutations is a common method for exploring key nucleotide sites of diseases in animal breeding. BioVenic uses the CRISPR genome engineering toolkit to introduce point mutations in animal genomes. This is accomplished through various methods, all of which are subject to rigorous quality management and quality assurance systems. These measures are in place to ensure that we meet the research requirements of our customers, particularly in the field of functional exploration of specific nucleotides.

Fig.1 Schematic Diagram of Point Mutation. (BioVenic Original)

Our Services

Point Mutation Strategy Customization

We help to design a CRISPR nuclease-mediated editing strategy for specific research requirement. BioVenic provides you with an effective plan for the point mutation project, using optimization algorithms to select target sites in genes, maximize editing activity, and minimize the probability of off-target effects, as well as the design of vectors and donor templates for mutations.

Point Mutation Vector Construction

BioVenic offers a comprehensive range of custom construction services for editing tools that are integrated into the genome of farmed animals. Our services include the design and synthesis of guide RNA, the construction of tool enzymes, and the creation of donor DNA expression vectors. Expression vectors are pre-tested to ensure their effectiveness prior to delivery.

Point Mutation Tools Delivery Solution

The components for point mutation editing need to be introduced into the cultured animal cells in order to function. If you encounter any challenges in this area, BioVenic offers tailored services utilizing the gene delivery solutions for animal breeding that have been developed and refined over the years.

Point Mutation Introduction Screening

Positive results will undergo screening by PCR and sequencing to identify site-directed mutations in the gene. Specifically, the site targeted by the nuclease is amplified using PCR, and the resulting PCR product is then sequenced to visualize any mutations that may have occurred.

Applications of Point Mutation

Types	Function	Applications
Silent Mutation	No effect as the encoded amino acids is not altered.	Codon optimization of heterologous genes.
Missense Mutation	Amino acid changes due to changing codons.	Functional exploration of key gene targets and corresponding proteins.
Nonsense Mutation	Protein truncation due to substitution of normal amino acid codons with stop codons.	Protein engineering, inactivation of target protein.

Why Choose Us?

Genomic Selection in Animal Breeding

We have extensive experience in overcoming point mutation problems in complex sequences such as hairpin structures, high GC, and polymeric structures.

Genome-wide Associated Studies (GWAS) in Animal Breeding

We provide one-stop upstream and downstream services, including template sequencing, de novo gene synthesis, construction and cloning of expression vectors, and pre-delivery sequencing verification.

Animal Genetic Modification

All mutated sequences are double-stranded DNA sequenced via BioVenic's high-throughput sequencing platform to achieve high accuracy.

BioVenic holds rich experience in point mutations in the research genome of farmed animals and customizes point mutation research programs to suit various research objectives. Our professional services ensure that your project is completed accurately, efficiently, and safely, allowing you to obtain mutant constructs quickly. Please feel free to contact us as long as you have requirements related to the introduction of point mutations.