Prime Editing in Precision Animal Breeding

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Prime Editing in Precision Animal Breeding

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BioVenic excels in the latest prime editing technology, relying on a highly skilled gene editing team and a comprehensive project development process. We offer customized services with a high level of professionalism and feasibility to meet customers' specific research editing requirements for farmed animal genomes.

Background of Prime Editing

The achievement of prime editing relies on two main components, including a prime editor that fuses the nuclease and the reverse transcriptase, and the pegRNA that combines the template and the guide.

Fig.1 Flowchart of Prime Editing Mechanism. Fig.1 Schematic Diagram of Prime Editing^1,2.

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Prime-editing Guide RNA (pegRNA) Design and Synthesis

The pegRNA consists of a fragment homologous to a genomic target sequence and a region to perform editing. It can be regarded as a specially modified sgRNA, whose sequence length and other designs not only affect the positioning of the editor, but also affect the editing efficiency. BioVenic undertakes the design and synthesis of the pegRNA to ensure that your prime editing process is performed accurately and efficiently.

Prime-editor (PE) Delivery Strategies

Most current in vivo studies are based on AAV delivery system due to its efficient cellular uptake, low immunogenicity, and a wide range of serotype specificities. However, the PE exceeds the cargo capacity of a single AAV carrier, making safe and efficient delivery challenging. Our professional R&D team is working to overcome the size limitations imposed by viral vectors and provide an appealing and safe method for the application of PE system to target cells.

Prime Editing with High Efficiency

Prime editing has been developed as a revolutionary new technology, and the efficiency of its editing is a crucial bottleneck for its application and advancement. By optimizing the pegRNA and improving PE enzyme proteins, BioVenic has developed a stable, efficient, and accurate editing system for animal breeding to ensure the delivery of high-quality results.

Fig.2 Prime Editing Process Flowchart. Fig.2 Flow Chart of Prime Editing Mechanism^1,3.

What Can PEs Do?

Prime editing is still in its infancy, and it is worth exploring and developing due to its specificity and editing potential. The primer editing system is a significant milestone in the development of universal methods for genome editing. It has less stringent requirements for PAMs due to the varying lengths of RT templates, and it does not involve non-target editing. It has broader development prospects than other editing methods for improving the genome editing and breeding of farmed animals.

Correction of functional gene in farmed animals.

Treatment of mono-genic or polygenic diseases in farmed animals.

Insertion of large DNA fragments in genome.

Improvement of genome editing flexibility and specificity.

Advantages of Prime Editing in Precision Animal Breeding

Service	Comparison	Advantages
Prime Editing	Genome Editing	Less limited by PAM sequence location. The PAM-to-edit distance can be more than 30 base pairs.
	Base Editing	More versatile and precise. Prime editing is even more precise, allowing for all 12 possible base-to-base changes.
	Homology Directed Repair	Less by-products and more efficiency. Unexpected indels and other editing results occur less frequently.

Why Choose Us?

One-stop service of Prime editing in farmed animal genome.

Efficient delivery system for editing components to animal cells.

Precise and flexible base-level genome editing.

Relying on a team of experts in the field of animal gene editing and a mature and professional technology platform, BioVenic has mastered this cutting-edge genome editing technology and provides stable and efficient project solutions for customer groups. We make prime editors easy to implement, advancing the research and development of precision animal breeding programs in a more flexible and safer manner. Please feel free to contact us if you have any related needs.

References

Zhao, Zhihan, et al. "Prime editing: advances and therapeutic applications." Trends in Biotechnology 41.8 (2023): 1000-1012.
Image retrieved from Figure 1 "CRISPR–Cas-mediated precise genome-editing systems". Zhao, Zhihan, et al., 2023, used under CC BY 4.0. The original image was modified by extracting and using only part C and the title was changed to "Schematic Diagram of Prime Editing".
Image retrieved from Figure 2 "Mechanism of prime editing". Zhao, Zhihan, et al., 2023, used under CC BY 4.0. The original image was modified by changing title to "Flow Chart of Prime Editing Mechanism".