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Prime Editing in Precision Animal Breeding
BioVenic excels in the latest prime editing technology, relying on a highly skilled CRISPR-oriented team and a comprehensive project development process. We offer customized services with a high level of professionalism and feasibility to meet customers' specific research editing requirements for farmed animal genomes.
Background of Prime Editing
Prime-editors (PEs) have emerged as a novel approach to expand base-level DNA editing beyond transition and transversion accomplished by Base Editors (BEs), but also the small mutation with deletion or insertion. The achievement of prime editing relies on two main components, including a prime editor that fuses the Cas9n protein and the reverse transcriptase, and the pegRNA that combines the template and the guide.
Fig.1 Schematic Diagram of Prime Editing1,2.
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Fig.2 Flow Chart of Prime Editing Mechanism1,3.
What Can PEs Do?
Prime editing is still in its infancy, and it is worth exploring and developing due to its specificity and editing potential. The primer editing system is a significant milestone in the development of universal methods for genome editing. It has less stringent requirements for PAMs due to the varying lengths of RT templates, and it does not involve non-target editing. It has broader development prospects than other editing methods for improving the genome editing and breeding of farmed animals.
Correction of functional gene in farmed animals.
Treatment of mono-genic or polygenic diseases in farmed animals.
Insertion of large DNA fragments in genome.
Improvement of genome editing flexibility and specificity.
Advantages of Prime Editing in Precision Animal Breeding
| Service | Comparison | Advantages |
|---|---|---|
| Prime Editing | CRISPR Genome Editing | Less limited by PAM sequence location. The PAM-to-edit distance can be more than 30 base pairs. |
| Base Editing | More versatile and precise. Prime editing is even more precise, allowing for all 12 possible base-to-base changes. | |
| Homology Directed Repair | Less by-products and more efficiency. Unexpected indels and other editing results occur less frequently. |
Why Choose Us?
One-stop service of Prime editing in farmed animal genome.
Efficient delivery system for editing components to animal cells.
Precise and flexible base-level genome editing.
Relying on a team of experts in the field of animal gene editing and a mature and professional technology platform, BioVenic has mastered this cutting-edge genome editing technology and provides stable and efficient project solutions for customer groups. We make prime editors easy to implement, advancing the research and development of precision animal breeding programs in a more flexible and safer manner. Please feel free to contact us if you have any related needs.
References
- Zhao, Zhihan, et al. "Prime editing: advances and therapeutic applications." Trends in Biotechnology 41.8 (2023): 1000-1012.
- Image retrieved from Figure 1 "CRISPR–Cas-mediated precise genome-editing systems". Zhao, Zhihan, et al., 2023, used under CC BY 4.0. The original image was modified by extracting and using only part C and the title was changed to "Schematic Diagram of Prime Editing".
- Image retrieved from Figure 2 "Mechanism of prime editing". Zhao, Zhihan, et al., 2023, used under CC BY 4.0. The original image was modified by changing title to "Flow Chart of Prime Editing Mechanism".