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Base Editing in Precision Animal Breeding
As an essential technical support for genome editing in farmed animals, BioVenic has incorporated base editing technology into its CRISPR gene editing services to fulfill customer-specific mutation research requirements.
Background of Base Editing
CRISPR-Cas-based base editors (BEs) are an emerging gene editing tool that have shown unlimited potential and prospects in disease correction and key targets inactivation in precision animal breeding. When the base editor fusion protein targets genomic DNA under the guidance of sgRNA, cytosine deaminase binds to the single-stranded DNA (ssDNA) in the R-loop region formed by Cas9 protein, sgRNA, and genomic DNA. This binding ultimately leads to base replacement. Base editors enable more precise complete genome modifications without causing double-strand breaks (DSBs) or homology-directed repair (HDR).
Benefits of the Base Editing
Multi-target editing with simultaneity, high-efficiency and accuracy.
No random mutation interference.
Optimization of targeted gene editing.
Improvement of viability and survival.
Fig.1 Schematic Diagram of Base Editing.1
Featured Services of Base Editing
BioVenic's R&D team has systematically mastered base editing technology, including its technical characteristics, development process, optimization, application, off-target effects, and improvement strategies. At the same time, we have conducted in-depth development, such as establishing new base editing systems, enhancing specificity at the genome-wide level, and optimizing the delivery form of base editing materials. Our commitment is to provide comprehensive customized base editing services for scientific research customers.
Applications of Base Editing in Animal Breeding
Base editing technology, as an emerging gene editing method, has partially addressed the limitations of traditional CRISPR genome editing in terms of accuracy and editing efficiency. There have already been successful cases of gene editing breeding through this technology. Zhou et al. utilized base editors (BEs) to introduce point mutations in the SOCS2 gene of the sheep genome, resulting in a stable increase in the growth rate. This demonstrates the potential of applying BE-mediated point mutations in animals to improve production traits in cultured breeds. Based on the precise characteristics of base editing, it can also be used to identify key amino acids in genome structure-function analysis, narrow down the scope of key targets, and enrich the detailed information of genomic targets related to desirable traits. Such base-level gene manipulation has guiding significance for the development of the entire gene editing breeding.
Why Choose Us?
Improved base editors include cytosine base editor (CBE) and adenine base editor (ABE).
Efficient and safe delivery of base editing tools.
Animal genome-wide base editing system with higher specificity and safety.
BioVenic's base editing services enable precise and controlled changes to target sequences, which help advance research and development in precision animal breeding research. Our expert team designs guide RNA for your gene of interest, precisely utilizes base editing to achieve non-damaging editing of base pairs, and ultimately achieves results with a higher cell survival rate and editing accuracy compared to traditional CRISPR technology. Please feel free to contact us to customize the base editing project according to the needs of your scientific research project.
References
- Zhao, Z.; et al. Prime editing: advances and therapeutic applications. Trends in biotechnology. 2023, 41(8): 1000–1012.
- Zhou S.; et al. Programmable Base Editing of the Sheep Genome Revealed No Genome-Wide Off-Target Mutations. Front. Genet. 2019, 10:215.