Veterinary Antimicrobial Resistance (AMR) Genotypic Characterization

Antimicrobial resistance happens when pathogens evolve to resist the effects of antimicrobial drugs. As these pathogens become resistant, treating infections in animals becomes more challenging. To address this critical problem, BioVenic is utilizing advanced genotypic characterization services. These services provide a detailed understanding of antimicrobial resistance by examining the genetic composition of pathogens. By analyzing specific genes or genetic mutations linked to resistance, researchers can uncover the mechanisms behind resistance, leading to better-informed decisions in developing treatments.

Veterinary AMR Genotypic Characterization Services

Equipped with advanced equipment and expertise in molecular biology and microbiology, our veterinary antimicrobial resistance genotypic characterization services provide a range of molecular techniques to identify and analyze genes associated with antimicrobial resistance in animal pathogens.

• PCR
  We offer PCR services to identify the presence of specific resistance genes in bacterial samples. Primers specific to known resistance genes are used to amplify these regions, providing a rapid and sensitive method for detection. Our quantitative PCR (qPCR) service allows for the quantification of resistance gene copies in a sample, offering insights into the gene's abundance and potential impact on resistance. Our multiplex PCR service can simultaneously amplify multiple resistance genes in a single reaction, increasing throughput and efficiency.
• Whole Genome Sequencing
  Our WGS service enables to identifying all known and novel resistance genes within a pathogen's genome. It is ideal to learn the genetic context of resistance genes, such as their location on plasmids or chromosomes, and their association with other genetic elements like integrons and transposons. Our WGS service follows comprehensive bioinformatic analysis, tracing the evolutionary history and transmission pathways of resistant strains through comparative genomics and uncovering the mechanisms of resistance by identifying mutations and gene variants associated with antimicrobial resistance.
• Targeted DNA Sequencing
  BioVenic's targeted DNA sequencing service focuses on sequencing known resistance genes or regions within the genome of veterinary pathogens. The service is more cost-effective than WGS when the goal is to analyze specific genes or regions. The targeted genes include flagella encoding genes, energy production enzymes, and other genes related to cell growth rate.
• DNA Microarray
  We provide customized DNA microarray services to screen for a wide array of resistance genes in a single assay and assess the expression levels of resistance genes under different conditions. Our service provides a comprehensive insight in gene expression patterns related to resistance and isolation of multiple resistance genes in pathogens.
• Gene Expression Studies
  Our gene expression service is based on our RNA-Seq platform and our experienced bioinformatic team. The service enables to reveal resistance genes expression and regulation in response to antimicrobial agents. Our bioinformatic analysis covers genetic networks, gene functions, and pathways that are involved in the regulation of resistance genes. Our service can profile the expression of resistance genes in different conditions and different antibiotics to develop potential targets for novel antimicrobial therapies for animals.

Service Workflow

BioVenic's veterinary AMP genotypic characterization services play a crucial role in the study of antimicrobial resistance, providing unique insights and capabilities. PCR offers rapid and sensitive detection, while WGS provides a comprehensive view of the genome. DNA microarrays allow for high-throughput screening, targeted sequencing focuses on specific genes, and gene expression studies reveal regulatory mechanisms. These tools enable a detailed and multifaceted understanding of AMR, which is essential for developing effective strategies to combat resistance and protect animal and public health. Please feel free to contact us to learn more about our genomic services in veterinary genotyping and analysis.