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Veterinary Vaccine In Vitro Efficacy and Potency Assays
BioVenic offers in vitro efficacy and potency assays for veterinary vaccines, assessing their ability to induce immune responses and prevent diseases. Utilizing cell culture to mimic host interactions, we provide stable and contamination-free cell lines to aid in vitro experiments. These experiments assist in screening the most promising candidates for veterinary vaccines, facilitating further evaluation in subsequent stages of development trials.
Our Services
ELISA (Enzyme-Linked Immunosorbent Assay)
The fundamental principle of ELISA involves the specific binding of enzyme-labeled antibodies or antigens to the target molecule under investigation (typically an antibody or antigen). Subsequent chemical reactions are employed to detect this binding, quantifying the presence of the target molecule. BioVenic provides ELISA services for veterinary vaccines to assist in measuring and analyzing antibody levels and animal immune responses. BioVenic also provides customized ELISA development services according to your specific requirements. We have perfect reagent formulations, enzyme labeling technology, and abundant antigen and antibody resources to ensure that the assay accurately meets your veterinary vaccine development goals.
ELISA is utilized to investigate the immunogenicity of different veterinary vaccine candidates or antigens, helping select the most effective vaccines.
ELISA is employed to determine specific antibody levels secreted by host cells, evaluating whether the veterinary vaccine induces a sufficient immune response.
ELISA can be used to determine if host cells are generating immune protection in response to your developed veterinary vaccine product.
ELISA is used to measure the concentration of antigens or pathogenic molecules in veterinary vaccines to ensure product quality and consistency.
Neutralization Assay
BioVenic provides the neutralization assay, used to assess the neutralizing effect of antibodies against a pathogen, specifically referring to the ability of antibodies or antiserum to prevent the pathogen from entering host cells or inhibit its replication, thereby reducing or preventing infection. Viruses must replicate and proliferate within live cells, and specific antiviral antibodies (neutralizing antibodies) bind to the virus, rendering it incapable of entering host cells, thus preventing the virus from replicating within host cells.
Fig.1 Virus neutralization assay workflow. (BioVenic Original)
| Target Virus and Host Cells Provision | BioVenic supplies the specific target virus strain and suitable host cells required for the assay. |
| Host Cell Treatment | The provided host cells are treated with your veterinary vaccine product. |
| Incubation | Treated host cells are mixed with the target virus, and the resulting mixture is incubated. |
| Observation of Cytopathic Effects | Observation of the antibody's impact on virus-induced cytopathic effects, including cell shrinkage, enlargement, degeneration/lysis, cell fusion, and the formation of inclusion bodies. |
| Evaluation of Plaque Formation | The ability of the antibodies to inhibit plaque formation is assessed during the incubation period. |
| Determination of Neutralizing Antibodies | The observed cytopathic effects and plaque formation provide insights into the production of neutralizing antibodies. |
| Reflecting Vaccine Efficacy | The presence and effectiveness of neutralizing antibodies reflect the efficacy of your veterinary vaccine. |
Antigen Titer Assay
BioVenic offers antigen titer testing services for veterinary vaccines, which assist you in evaluating the antigen concentration to ensure that your vaccine contains a sufficient amount of antigen material to elicit the desired immune response.
| Dilution | Dilutes veterinary vaccine into a series of different concentrations using buffer solutions or culture media. |
| Mixing with Antibodies | The diluted vaccine samples are mixed with corresponding known concentrations of antibodies. |
| Flow Cytometer Analysis | Using fluorescent markers, we label the formed antigen-antibody complexes. |
| The labeled complexes are analyzed using a flow cytometer to measure fluorescent signals after a designated reaction period. | |
| Antigen Titer Determination | Comparative analysis of fluorescent signal intensities allows us to determine antigen titers in cells at different concentrations. |
| Subsequent data analysis involves plotting a standard curve to establish the relationship between antigen concentration and fluorescent signals. | |
| Based on the fluorescent signal in the cells, we determine the antigen titer using the established standard curve. |
Cytokine Assay
Cytokines is a collective term for biologically active small protein molecules secreted by cells, including interferons, interleukins, chemokines, and more. Cytokine assays are employed to measure whether veterinary vaccines can stimulate immune cells to produce cytokines, aiding in the induction of immune responses. Throughout the immune response process, they play a crucial role in immune regulation and inflammatory reactions, among other processes. BioVenic has established an advanced laboratory platform with various detection methods such as ELISA, flow cytometry, and Luminex for you to choose from.
Related Veterinary Vaccine Assays
The results of in vitro efficacy and potency assays serve as a reference for subsequent in vivo experiments, ultimately aimed at protecting animals from specific diseases. BioVenic also offers other assays related to veterinary vaccines, both in vivo and in vitro. Tailored to specific needs, we provide customized assay combinations. If you are interested in these assays, please click on the link below.
Why Choose Us?
Versatile in vitro potency and potency assays to visualize the efficacy and immunogenicity of veterinary vaccines.
Customized combinations of in vitro potency and potency assays for veterinary vaccines according to specific research and development needs.
Rapid delivery of highly accurate in vitro efficacy and potency measurements for veterinary vaccines.
The purpose of BioVenic's provision of in vitro efficacy and potency testing services for veterinary vaccines is to determine whether the active components within the vaccine are potent enough to elicit a robust immune response. If your veterinary vaccine requires in vitro efficacy and potency testing, please feel free to contact us, we are dedicated to serving your needs!