This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
Animal Microbiome Pan-gemome Analysis
BioVenic utilizes pan-genome analysis of animal microbiomes to acquire more detailed and comprehensive information on microorganisms. By correlating phenotypes with functional genes or sequences in diverse genomes, we identify valuable genetic resources for scientific researchers to enhance animal genetics through genetic improvement. We support research and analysis on the evolution, adaptability, and population structure of microbiome strains. We continuously optimize the relationship between the bacterial community and the animal host to enhance animal nutrition and metabolism, animal genetics, animal immunity and health.
Background
The pan-genome captures the complete genetic content of a species, encompassing the core genome responsible for the species' major phenotypic characteristics and the variable genome linked to the species' genetic diversity and individual uniqueness. Pan-genome sequencing utilizes high-throughput sequencing and biological information analysis methods to sequence and assemble pan-genomes of various subspecies/individual materials. This process aims to construct a pan-genome map and enhance the genetic information of the species. Pan-genome sequencing can not only acquire multiple genomes and enhance the gene set of the species but also capture unique DNA sequences and functional gene information for populations and even individuals. This lays the groundwork for phylogenetic analysis and functional biology research, advancing in animal microbiome related research.
Fig.1 Microbiome Analysis Using Pan-genome Studies of Bacillus Species. 1
Animal Pan-Genomic Technology Platforms
- De novo Assembly NOT Based on Reference Genome
BioVenic provides the most classic method for constructing a pan-genome or reference genome. It assembles and annotates multiple individuals from scratch, and then compares the assembled sequence of each individual with the reference genome sequence to identify differences in the alignment. These individual genes are variable and unique.
- Reference Genome-based Iterative Assembly
BioVenic can initially align the reads of each individual to the animal microbiome reference genome, and subsequently identify the unaligned segments for assembly to acquire new gene sequences and extend the original reference sequence. Iterate step by step through the processing of all individuals until the pan-genome is established and annotated. This method requires a lower sequencing depth because it can pool individuals with closer genetic types together for iterative assembly, thereby increasing the depth of the assembly.
- De novo Assembly Based on Reference Genome
BioVenic provides the "map to pan" method for pan-genome sequencing. This method first performs de novo assembly and then aligns the assembled gene sequences of each individual to the reference genome to identify unaligned sequences. All unaligned sequences were then identified, and a pan-genome was constructed. This method is also more suitable for large-scale microbial groups. BioVenic provides a wide range of computer resources to fulfill customers' detection and analysis requirements.
Fig.2 Principal Diagram of Animal Pan-genome Technology Platform.
Applications of Animal Pan-Genome
| Objects | Goals | |
|---|---|---|
| Gut Microbiome Pan-genome Analysis | Microbial communities in animal gut and feces samples. |
|
| Skin Microbiome Pan-genome Analysis | Microbial communities in the skin. |
|
| Oral Microbiome Pan-genome Analysis | Supragingival plaque, tongue dorsum, and other sites in the oral. |
|
| Bladder Microbiome Pan-genome Analysis | Microbial communities in the vaginal and bladder. |
|
Why Choose Us?
A one-stop omics solution has been developed specifically for analyzing and detecting the microbiome pan-genome in animal samples.
Diversified microbiome pan-genome analysis services meet the extensive research needs of scientific research groups.
Support advanced bioinformatics data analysis solutions customized to meet the specific requirements of research projects.
BioVenic offers pan-genomic analysis services for animal microbiomes, encompassing a diverse array of animal samples and scientific research fields. Relying on our extensive experience in large-scale projects, we utilize a combination of an automated data statistical analysis platform and manual proofreading. With guidance and support from experts in their fields, our goal is to efficiently generate high-quality, publishable data to enhance your research. If you have any questions about the animal microbiome, whether regarding experimental design or technical aspects, please feel free to contact us.
Reference
- Kim, Yihwan, et al. "Pan-genome analysis of Bacillus for microbiome profiling." Scientific reports 7.1 (2017): 10984.