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Animal Microbiome Shotgun Metagenomic Sequencing and Analysis
BioVenic specializes in providing customized shotgun metagenomic solutions for animals and related samples to scientific researchers worldwide. These solutions enable the classification of microorganisms in animal samples at the species level and facilitate functional analysis. At the same time, we utilize multiple read sequencing techniques to efficiently identify less prevalent microbial groups, thereby achieving comprehensive species-level detection.
Background
Shotgun metagenomic customization solutions for animal microbiomes are primarily used to sequence and analyze the entire genome and functionally dissect the microbial communities present in animals and their associated environmental samples. Unlike detection technologies that only sequence specific conserved regions, such as 16S rRNA, this more comprehensive sequencing enables a more in-depth and detailed exploration of multiple microorganisms in the sample through whole-genome sequencing simultaneously. Shotgun metagenomic sequencing has become an important tool for studying microbial population structure due to its versatility and broad applicability. It can be used to analyze the intestinal microbiome, as well as other body parts such as the oral or nasal cavities, and environmental samples.
Fig.1 Microbiome Shotgun Metagenomic Sequencing to Detect Intestinal Microbial Diversity. 1
Animal Microbiome Shotgun Metagenomic Sequencing Services
BioVenic offers shotgun metagenomic sequencing and analysis services for animal samples. These services are used to identify different types of microorganisms and their corresponding functions. We divide the DNA in the sample into many small fragments for sequencing and then apply bioinformatics to analyze and integrate the sequencing results. This allows us to thoroughly analyze and gain a comprehensive understanding of microorganisms and their genetic potential in samples from animals and their respective environments.
- Sample Preparation and QC
BioVenic's laboratory team has developed comprehensive protocols for the collection and storage of animal samples for microbiome analysis. These protocols are crucial in ensuring accurate, reliable, and reproducible results in genetic sequencing. We develop customized collection and DNA extraction protocols based on the type of sample being analyzed and the specific objectives of scientific research. With our extensive project experience and state-of-the-art instruments and equipment, we accurately ensure the three crucial parameters of sterility, temperature, and time to maintain sample integrity.
- Library Construction
BioVenic offers library preparation services for shotgun metagenomic sequencing of animal microbiomes. This includes DNA fragmentation, indexing adapters and DNA ligation, as well as DNA cleaning to remove impurities in labeling reagents.
- Sequencing
BioVenic has established a high-throughput sequencing platform for DNA fragments. The sequencing services we offer combine multiple samples in a single sequencing run, allowing for high sequence coverage of each sample. This enables the detection of very low-abundance members of a microbial community and the identification of the microbial species to which they belong. We customize and optimize the sequencing technology process based on the specific sequence length and throughput in the customer's animal microbiome sample.
- Data Analysis
Our analytical data services identify and classify microorganisms present in samples. BioVenic utilizes a proprietary animal bioinformatics processing program to eliminate read and sequencing errors, clean the sequencing data, and subsequently compare it with established databases.
| Species Level Analysis |
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| Functional Level Analysis |
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Flow Chart of Animal Microbiome Shotgun Metagenomic Sequencing Solutions
Animal Microbiome Sequencing Methods Comparison
| Methods | 16S rRNA | Shotgun Metagenomic |
| Microbial Coverage | High | Limited |
| Taxonomic Resolution | Genus - species | Species-strain |
| Host Interference | Hardly | High |
| Functional Analysis | No | Yes |
| Database | Relatively complete | Still needs improvement |
| Bioinformatics Requirements | Lower | Higher |
Why Choose Us?
Assess bacterial diversity in animals and their associated environmental samples and detect the abundance of microorganisms in the environment.
Cross-joint applications in multiple fields such as microbiomes, animal physiology, and gene sequencing.
Project design and customized services covering a variety of scientific research needs.
A one-stop solution for studying non-culturable microorganisms that simultaneously performs microbial classification and functional analysis.
BioVenic offers customized shotgun metagenomics analysis services for scientific researchers conducting animal microbiome research. We specialize in high-resolution sequencing of the entire genome. Our comprehensive service includes standardized sample preparation and gene extraction processes, rigorous quality control, state-of-the-art sequencing platforms, and extensive bioinformatics expertise. These tools comprehensively and fully support customers in conducting relevant research on the microbial population structure, gene functional activity, mutual cooperation, and the relationship with the environment in different parts of animals. Please feel free to contact us for further information and to inquire about pricing.
Reference
- Ellegaard, Kirsten M., and Philipp Engel. "Beyond 16S rRNA community profiling: intra-species diversity in the gut microbiota." Frontiers in microbiology 7 (2016): 1475.