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Veterinary Virulence-associated Gene Discovery
Virulence is simply defined as the harm caused by a pathogen to its host, particularly in terms of the host's mortality. Virulence is a complex trait determined by a combination of pathogen, host, and environmental factors. Virulence-associated genes (VAGs) are nucleotide sequences within the pathogen's genome that contribute to the pathogenicity of the virus or its ability to cause disease in the host organism. BioVenic is committed to advancing research in veterinary virology. Through our veterinary virulence-associated gene discovery service, built upon advanced platforms, we assist clients in unraveling the mysteries surrounding the virulence of veterinary viruses.
Featured Veterinary Virulence-associated Gene Discovery Services
Distinguishing itself from traditional methods such as transposon mutagenesis and identification of upregulated genes within the virus, our services utilize advanced genome-wide association studies (GWAS) and comparative genomics to aid clients in discovering VAGs in veterinary viruses. We employ suitable nucleic acid extraction methods based on the type of veterinary virus, enabling the extraction of viral DNA or RNA from tissues, biological fluids, and various other sample types. Conducting WGS on both pathogenic and non-pathogenic virus genomes submitted by clients is a crucial step in helping identify VAGs, providing comprehensive information such as complete gene sequences and SNPs. Tailoring our services to the research focus and objectives of each client, we offer several distinctive service components.
Genome-wide Association Studies (GWAS)
Utilizing GWAS, we scan for SNPs and copy number variations (CNVs) between pathogenic viruses and control groups, conducting correlation analyses between genotypes and phenotypes. By identifying gene variations associated with virulence differences, we uncover potential gene regions linked to viral pathogenicity. GWAS is a large-scale analytical method aiding clients in identifying common genetic variations that may contribute to complex pathogenicity. Moreover, we adjust significance thresholds based on client requirements to expand or narrow down the potential range of VAGs.
Comparative Genomics
Through comparative genomics, we analyze the sequences, structures, and functions of genomes from multiple individuals of the same species (with varying virulence) or viruses across different species. Comparative genomics allows clients to gain insights into critical aspects of viral virulence, including entry mechanisms, replication strategies, and immune evasion strategies.
VAGs Validation and Functional Studies
We validate candidate virulence-associated genes identified through comparative genomics and GWAS using experimental methods such as qPCR, RT-PCR, or functional assays. Importantly, we conduct in vitro and in vivo studies to characterize the roles of selected genes in virus replication, host immune responses, and disease mechanisms.
Bioinformatics and Pathway Analysis
Bioinformatics and pathway analysis are crucial in unraveling the intricate molecular interactions and functional relationships among identified genes. We employ advanced bioinformatics analysis methods to provide actionable insights into pathways influencing viral virulence, thereby discovering VAGs from various perspectives.
Applications
Development of Antiviral Drugs Targeting Veterinary VRGs
We facilitate the development of antiviral drugs specifically targeting genes associated with veterinary viruses, contributing to advancements in veterinary medicine.
Analysis of the Pathogenic Mechanisms of Veterinary Viruses
The analysis of veterinary viruses' pathogenic mechanisms, provides valuable insights into how these viruses cause diseases and helps in the development of targeted interventions.
Identification and Characterization of Veterinary Viruses
Our services support the identification and characterization of veterinary viruses, aiding veterinary researchers in understanding the nature of viral infections.
Facilitation of Research on the Evolution of Virulence in Viral Populations
We contribute to the study of the evolutionary dynamics of viral virulence populations, offering key information on how virulence traits evolve.
Why Choose Us?
Specialized Knowledge
Our virus research team consists of experts with extensive experience in virology, genomics, and bioinformatics, ensuring comprehensive VAGs discovery for our clients.
Advanced Platforms
Utilizing state-of-the-art NGS platforms, we accurately detect the complete genomic sequences of two or more viruses provided by clients, guaranteeing reliable results for subsequent analyses.
Custom Solutions
We provide fully customized VAG discovery services by combining GWAS and comparative genomics, meeting the specific needs of research projects and ensuring optimal outcomes.
The continual emergence of high-quality genomes enhances the effectiveness of GWAS and comparative genomics as powerful tools for discovering virulence factors in veterinary viruses. BioVenic, with its dedicated technical team, is committed to helping clients discover all genes closely related to viral virulence through its custom veterinary virulence-associated gene discovery services. If you are interested in collaborating with us, please feel free to contact us at any time.
References
- Lau, Wing Yin Venus, et al. "Pathogen-associated gene discovery workflows for novel antivirulence therapeutic development." EBioMedicine 88 (2023).
- Stubben, Chris J., et al. "Steps toward broad-spectrum therapeutics: discovering virulence-associated genes present in diverse human pathogens." BMC genomics 10.1 (2009): 1-9.