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Veterinary Bacterial Pathogen 16S Amplicon Sequencing
Bacteria are widely present in various environments such as soil, water, air, and living organisms, posing potential threats to animal health. Some of these bacterial pathogens trigger various diseases, including tuberculosis, foot-and-mouth disease, and avian influenza, causing harm to livestock, companion animals, and poultry. Rapid identification techniques like 16S amplicon sequencing are crucial in slowing down the spread and impact of bacterial pathogens. As an innovative biotech company, BioVenic provides customers with a state-of-the-art veterinary bacterial pathogen 16S amplicon sequencing solution, utilizing advanced equipment to help quickly and accurately identify the types of bacteria or ancient bacteria infecting animals.
Featured Veterinary Bacterial Pathogen 16S Amplicon Sequencing Services
16S rRNA is a small RNA molecule found on the 30S subunit within the cells of bacteria and archaea. It exhibits high conservation within biological organisms, while also displaying certain differences among different species. We leverage these differences to assist customers in identifying and distinguishing various types of bacteria and archaea. With the capability to provide a comprehensive 16S rRNA sequencing service for diverse customer samples, including sample preparation, DNA extraction, sequencing, and data analysis, our featured services are outlined as follows.
Fig. 1 Process for identifying bacterial pathogens in animals using 16S rRNA sequencing.1
Bacterial DNA Extraction
We offer professional DNA extraction services tailored to bacterial suspensions, animal tissue samples, fecal specimens, blood samples, and various other biological samples. To ensure the extraction of high-quality DNA while eliminating contaminants, we conduct meticulous pre-treatment. Depending on the sample type, we select suitable DNA extraction kits and employ a combination of chemical and physical methods to provide customers with high-fidelity DNA extraction.
Library Construction
Library construction is a pivotal step in the 16S rRNA amplicon sequencing process. We employ professionally designed primers to perform PCR amplification of the V3-V4 region of the 16S rRNA gene. Through services such as end repair, A-tailing, adapter ligation, and size selection, we prepare libraries that meet the requirements of high-throughput sequencing for our customers. We provide transparent reports, including initial DNA quantity, library quality, and size selection results.
16S Sequencing
Tailoring our approach to the specific goals or requirements of our clients, we choose an appropriate sequencing platform for 16S sequencing. During the sequencing process, the DNA library undergoes random amplification, generating raw sequence data. We offer a range of sequencing platform options, including Sanger sequencing, Illumina, Ion Torrent, Oxford Nanopore, and PacBio, ensuring flexibility and compatibility with diverse research needs.
Data Analysis
Following the assembly of raw sequence data obtained from sequencing, we offer clients a variety of optional analysis projects. We assist clients in conducting bioinformatics analyses of the sequence data, including sequence alignment, species annotation, and diversity analysis. We are committed to providing customers with comprehensive and in-depth data interpretation.
Veterinary Bacterial Pathogen 16S Amplicon Sequencing Workflow
Applications
Why Choose Us?
Accurate Bacterial Identification
We use advanced 16S rRNA sequencing technology to accurately identify the types and quantities of veterinary bacterial pathogens for our customers.
Custom Data Analysis
We provide custom data analysis services, including sequence alignment, species annotation, diversity analysis, etc., to meet customers' unique research needs.
High-throughput Sequencing
Compared with traditional bacterial identification technology, 16S rRNA sequencing does not require culture and process many samples simultaneously, saving customers research time.
16S sequencing is one of the most widely used methods for rapidly identifying bacterial pathogens. BioVenic provides customers with a one-stop veterinary bacterial pathogen 16S amplicon sequencing solution to help customers accurately identify bacterial or archaeal species in samples. If you have any questions or require custom services, please feel free to contact us.
References
- Jahan, Nusrat A., et al. "Nanopore-based surveillance of zoonotic bacterial pathogens in farm-dwelling peridomestic rodents." pathogens 10.9 (2021): 1183.
- Clarridge III, Jill E. "Impact of 16S rRNA gene sequence analysis for identification of bacteria on clinical microbiology and infectious diseases." Clinical microbiology reviews 17.4 (2004): 840-862.