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Animal Feed Mycotoxin Analysis

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Animal Feed Mycotoxin Analysis Analytical Techniques Workflow Why Choose Us?

BioVenic provides a comprehensive selection of mycotoxin analysis services and products tailored for samples of animal feed, animal tissue, and animal-derived food, facilitating both qualitative and quantitative detection of various prevalent feed mycotoxins. This enables effective monitoring of mycotoxins and the formulation of mycotoxin-associated solutions. Our primary focus lies on aflatoxins, ochratoxins, fumonisins, trichothecenes, and zearalenone. Our testing arsenal encompasses immunoassays along with a diverse array of chromatographic methodologies.

Animal Feed Mycotoxin Analysis

For Animal Feed Mycotoxin Analysis, diverse approaches are employed to tackle the unique characteristics of each mycotoxin type. Our solutions for animal feed mycotoxin analysis offer precise and streamlined testing mechanisms across a broad spectrum of mycotoxin varieties.

  • Animal Feed Aflatoxins Analysis

Aflatoxins, lethal secondary metabolites produced by specific fungal strains like Aspergillus flavus and Aspergillus parasiticus, are categorized into B1, B2, G1, and G2, with B1 being the most potent. Our standard analytical techniques include TLC, HPLC, and ELISA.

  • Animal Feed Ochratoxins Analysis

Ochratoxins (OTs) constitute a group of mycotoxins generated by Aspergillus and Penicillium species. The presence of OTA in animal feed can detrimentally affect the health and productivity of farm animals, potentially resulting in OTA contamination in animal-derived products. Our analysis methods for OTs typically involve TLC, HPLC, LC-MS, and immunoassays, ensuring comprehensive coverage for accurate detection and monitoring.

  • Animal Feed Fumonisins Analysis

Fumonisins primarily stem from various Fusarium species and are frequently found in corn and its byproducts, posing a significant health risk to animals. Our analysis of fumonisins involves a range of chromatographic techniques, including TLC, GC, HPLC, LC-MS, alongside immunoassays like ELISA and LFD.

  • Animal Feed Trichothecenes Analysis

Trichothecenes, a family of over 200 toxins, share a common tricyclic 12,13-epoxytrichothec-9-ene (EPT) core structure. Among them, Deoxynivalenol, T-2, and HT-2 toxins are the most commonly reported. Our analytical methods for trichothecenes encompass TLC, HPLC, GC, and immunoassays to ensure thorough detection and analysis.

  • Animal Feed Zearalenone Analysis

Zearalenone is frequently found in various cereals like wheat, barley, maize, sorghum, rye, rice, corn silage, sesame seed, hay, flour, malt, soybeans, beer, and corn oil. It is associated with hyperestrogenism and reproductive disorders in cattle, swine, and poultry, with pigs being particularly vulnerable. The standard analytical methods used for zearalenone and its metabolites include LC-based techniques and immunoassays like ELISA and LFD, aiding in accurate analysis and detection.

  • Animal Feed Patulin Analysis

Patulin is commonly found in apples, apricots, grapes, grapefruit, peaches, pears, olives, and a range of cereal grains. This mycotoxin is known to exhibit genotoxic, reprotoxic, embryotoxic, and immunosuppressive properties. Our services for analyzing patulin in animal feed involve utilizing immunological techniques like ELISA and chromatographic methods such as HPLC-UV, LC-MS/MS, and GC-MS/MS.

Animal Feed Mycotoxin Analytical Techniques

The process of detecting mycotoxins typically involves three key stages: extraction, clean-up, and determination. We specialize in tailored mycotoxin testing solutions for various sample types, utilizing a range of cutting-edge detection technologies, including but not limited to the following methods.

  • Animal Feed Mycotoxin Immunoassays

Immunoassay techniques are built on antibody-antigen interactions. Our advanced immunosorbent assays offer swift and dependable tests with heightened sensitivity and specificity at a competitive cost. Our main mycotoxin immunoassays prominently feature ELISA and LFDs.

Enzyme-Linked Immunosorbent Assay (ELISA)

ELISA stands out as a pivotal tool for rapid mycotoxin detection and quantification. This method relies on the interaction between antigen-antibody complexes and chromogenic substrates, with results measured spectrophotometrically based on developed color. Our ELISA tests cater to diverse sample types.

Lateral-Flow Devices (LFDs)

Utilizing the competitive lateral flow immunoassay, LFDs excel in detecting low-molecular-weight compounds, including mycotoxins. With notable conveniences in speed and simplicity, LFDs are particularly effective for on-the-spot monitoring of mycotoxin presence in animal feed.

  • Animal Feed Mycotoxin Chromatographic Techniques

This set of methods aims at the quantitative determination of mycotoxins, employing liquid chromatography (LC) or gas chromatography (GC) linked to ultraviolet (UV), fluorescence (FLD), or mass spectrometric (MS) detection. Through sophisticated instrumentation and extensive sample preparation, these techniques ensure the accurate detection of a wide array of mycotoxins with optimal sensitivity.

Thin-Layer Chromatography (TLC)

TLC serves as a potent, rapid, and often cost-effective separation technique in mycotoxicology, particularly useful for multi-mycotoxin analyses and the examination of structurally related or individual mycotoxins.

GC and GC-MS

GC is harnessed as an analytical tool for mycotoxins that can be volatilized in a heated GC column or possess specific functional groups conducive to conversion into volatile derivatives. Coupling with a mass spectrometer enhances specificity in detection, identification, and determination of mycotoxins.

High-Performance Liquid Chromatography (HPLC)

Known for its high resolution and increasing automation, HPLC employs various detectors like fluorescence (FLD), UV-visible (UV), photodiode array, and MS to analyze mycotoxins efficiently.

Liquid Chromatography-Mass Spectrometry (LC-MS)

The synergy between chromatography and MS elevates the analytical capacities of both techniques. The LC-MS system, especially the LC-tandem MS (MS/MS), proves instrumental in precise multimycotoxin determination, offering superior sensitivity, specificity, and reliability, particularly for trace-level mycotoxin analysis.

Animal Feed Mycotoxin Analysis Workflow

Fig. 1 Animal feed mycotoxin analysis workflow (BioVenic Original)

Why Choose Us?

Our state-of-the-art analysis platform boasts a diverse array of advanced mycotoxin testing instruments and products, ensuring swift and precise detection services for mycotoxins in animal feed.

We cater to a broad spectrum of prevalent mycotoxins and can customize our testing technology to suit your sample type and specific requirements for optimal results.

With a wealth of expertise in animal nutrition, we are well-equipped to bolster animal health and nutrition through meticulous mycotoxin testing.

By synergizing our internal expertise and external resources, BioVenic delivers comprehensive mycotoxin analysis services for animal feed, animal tissues, and animal-derived foods, all under one roof. Our repertoire includes a wide range of immunoassays and chromatographic techniques to pinpoint key mycotoxins that impact the well-being and development of animals, such as aflatoxins, ochratoxins, fumonisins, trichothecenes, and zearalenone. Contact us if you are intrigued by our Animal Feed Mycotoxin Analysis services; we are eager to grasp your testing requisites and offer swift and precise mycotoxin testing services tailored to your needs.

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