CRISPR gRNA Design in Precision Animal Breeding

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CRISPR gRNA Design in Precision Animal Breeding

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Guide RNAs (gRNAs), as targets for recognition, can be likened to the GPS system in CRISPR. It combines with the activated Cas protease to complete the cleavage or modification of the downstream target at the PAM site, thereby facilitating the efficient and precise genome modification process. With the experienced synthetic biology research team, BioVenic designs custom gRNA sequences tailored to your specific genome editing requirements. We offer a range of editing services and aim to be your reliable partner on your scientific research journey.

Fig.1 Visual Representation of CRISPR gRNA Composition. (BioVenic Original) Fig.1 Schematic Diagram of CRISPR gRNA Design. (BioVenic Original)

Custom gRNA Design Services

gRNA Design in Precision Animal Breeding

gRNA is one of the key tools in the entire editing process, serving as a guide and positioning tool. BioVenic assists in selecting editing site and gRNA design, overcoming obstacles that may arise when applying CRISPR technology, which aim to enhance the understanding of the molecular mechanisms that are associated with important traits in farmed animals, including growth, production yield and quality, reproduction, disease resistance, and overall health.

Minimizing the Off-target Rate

Our gRNA design services focus on the base complementation match between the N20 sequence of the gRNA sequence and the animal genome sequence, which minimize the off-target rate, maximize the accuracy of editing and reduce the generation of undesired mutations.

Designing a Genome-wide gRNA Library

With the high-throughput gene synthesis platform and extensive expertise in CRISPR technology, BioVenic help our customers design and construct genome-wide gRNA libraries of farmed animals based on your specific requirements, which enable to edit the target gene in the whole genome and achieve high-throughput functional gene screening.

Fig.2 Guide RNA Design Service Process in CRISPR. (BioVenic Original)

Principles in the Design of sgRNA

Does not affect other genes, particularly those that encode proteins.
Aim to impact all transcripts as extensively as possible.
The knockout gRNA fragment is designed within the intron to eliminate the entire exon region and prevent the translation of any residual proteins.
When designing gRNA, comprehensive consideration must be given to the sequence, position, positive and negative strands, GC content, potential off-target sites, and other information of the candidate editing site.

Why Choose Us?

We perfectly meet the basic research needs of the vast majority of animal genome editing.

Our custom-designed gRNAs are highly suited for farmed animal genome editing.

Diversified plasmid structures for gRNA delivery are available for customers to choose according to their projects.

Multiple gRNAs are simultaneously designed for the editing target and the one with the highest editing efficiency is selected for delivery.

BioVenic has an experienced team of CRISPR genome editing experts specializing in precision animal breeding. We provide you with the necessary gRNA design for individual editing sites or specific metabolic pathways, as well as genome-wide gRNA library design. According to a thorough analysis of your specific project, we meticulously design the optimal gRNA sequence and deliver it using the delivery method of your choice. Please feel free to contact us for more details and pricing inquiries.